Application
Western blot analysis of Cytokeratin 23 expression in JAR (A) and HeLa (B) whole cell lysates and HeLa nuclear extract (C).| Product Name | Cytokeratin 23 Antibody (H-130) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 161-290 mapping within an internal region of Cytokeratin 23 of human origin |
| Modification Notes | Cytokeratins comprise a diverse group of intermediate filament proteins that are expressed in both keratinized and non-keratinized epithelial tissue. The Cytokeratin proteins play a critical role in differentiation, as well as tissue specialization and function, and maintenance of the overall structural integrity of epithelial cells. There are two types of Cytokeratins, namely the type I Cytokeratins and the type II Cytokeratins. Cytokeratin 23, also known as KRT23, K23, CK23 or HAIK1, is a 422 amino acid intermediate filament protein that functions as a heterotetramer that is composed of two type I and two type II Cytokeratins. Characteristic of most Cytokeratins, Cytokeratin 23 is thought to participate in maintaining the structural integrity of a variety of cells. Cytokeratin 23 expression is induced in pancreatic cancer cells, suggesting a possible role in carcinogenesis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Cytokeratin 23 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 161-290 mapping within an internal region of Cytokeratin 23 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 161-290 mapping within an internal region of Cytokeratin 23 of human origin |
Application
Western blot analysis of Cytokeratin 23 expression in JAR (A) and HeLa (B) whole cell lysates and HeLa nuclear extract (C).| Application Notes | recommended for detection of Cytokeratin 23 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
