Application
Western blot analysis of CRIF1 expression in mouse heart tissue extract (A) and F9 whole cell lysate (B).| Product Name | CRIF1 Antibody (M-222) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-222 representing full length CRIF1 of mouse origin |
| Modification Notes | CRIF1, also known as GADD45GIP1, PLINP1, PRG6 or CKBBP2, is a 222 amino acid nuclear protein that plays a role in apoptosis control. Expressed in a variety of tissues, including heart, thyroid, trachea, kidney, ovary, pancreas, testis and stomach, CRIF1 functions as a negative regulator of G1 to S phase cell cycle production, specifically by working with GADD 45 proteins to inhibit the activity of cyclin-dependent kinases (Cdks). While overexpression of CRIF1 results in cell cycle arrest at the G1 phase, downregulation of CRIF1 by p53 in apoptotic cells promotes cell cycle progression and may be an important factor in tumor growth and metastasis. CRIF1 is subject to phosphorylation by casein kinase II, an event that is thought to decrease CRIF1 activity and promote cellular proliferation. Human CRIF1 shares 90% homology with its mouse counterpart, suggesting a conserved role between species. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CRIF1 of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-222 representing full length CRIF1 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-222 representing full length CRIF1 of mouse origin |
Application
Western blot analysis of CRIF1 expression in mouse heart tissue extract (A) and F9 whole cell lysate (B).| Application Notes | recommended for detection of CRIF1 of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
