Application
Western blot analysis of DDX25 expression in mouse brain tissue extract.| Product Name | DDX25 Antibody (H-40) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 439-478 mapping near the C-terminus of DDX25 of human origin |
| Modification Notes | DDX25, also designated GRTH for gonadotropin-regulated testicular RNA helicase, is a testis-specific member of the DEAD-box protein family found in Leydig and germ cells. DDX25 has both ATPase and RNA helicase activity, regulating such translational-associated events as mRNA nuclear export during spermatid development. DDX25 can be phosphorylated on threonine residues, with the phosphorylated form found only in the cytoplasm and not in the nucleus. Essential for spermatogenesis, DDX25 participates in germ cell development and is up-regulated by gonadotropin at the transcriptional level. Genetic variations in DDX25 may contribute to male infertility due to spermatogenic impairment. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of DDX25 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 439-478 mapping near the C-terminus of DDX25 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 439-478 mapping near the C-terminus of DDX25 of human origin |
| Entrez Gene | 29118 |
|---|
Application
Western blot analysis of DDX25 expression in mouse brain tissue extract.| Application Notes | recommended for detection of DDX25 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
