Application
Western blot analysis of granzyme M expression in non-transfected 293T: (A), human granzyme M transfected 293T: (B) and WI 38 (C) whole cell lysates.| Product Name | granzyme M Antibody (H-70) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 71-140 mapping within an internal region of granzyme M of human origin |
| Modification Notes | The granzyme family of proteins belong to the larger peptidase S1 family. Granzyme A and granzyme B are serine proteases that facilitate apoptotic signaling in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Within the granules of activated CTLs, granzyme A and granzyme B are processed and converted to their active forms by the lysosomal cysteine protease cathepsin C. Once cleaved, these active proteases target distinct substrates for proteolysis, thereby mediating apoptosis through two different pathways. Granzyme H localizes to cytoplasmic granules of cytolytic T lymphocytes and is important for target cell lysis in cell-mediated immune responses. Granzyme K is a serine protease localizing to the granules of NK cells and cytotoxic T lymphocytes. Granzyme M (also designated NK cell granular protease, HU-Met-1 or met-1 serine protease) contains one peptidase S1 domain. Granzyme M is a Trypsin-fold serine protease that localizes to granules of large granular lymphocytes (NK cells) and cleaves peptide substrates after leucine, norleucine or methionine. This enzyme may play a role in target cell death induction by cytotoxic lymphocytes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of granzyme M of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 71-140 mapping within an internal region of granzyme M of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 71-140 mapping within an internal region of granzyme M of human origin |
| Entrez Gene | 3004 |
|---|
Application
Western blot analysis of granzyme M expression in non-transfected 293T: (A), human granzyme M transfected 293T: (B) and WI 38 (C) whole cell lysates.| Application Notes | recommended for detection of granzyme M of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
