Application
Western blot analysis of WNK1 expression in T-47D (A), A-375 (B) and HeLa (C) whole cell lysates and mouse kidney tissue extract (D).| Product Name | WNK1 Antibody (F-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of WNK1 of mouse and human origin by WB, IP and ELISA |
| Modification Notes | WNK1 (WNK lysine deficient protein kinase 1), also known as KDP (kinase deficient protein), PSK, erythrocyte 65 kDa protein (p65), HSN2, HSAN2 or PRKWNK1, is a 2,382 amino acid cytoplasmic protein that participates in cell signaling, proliferation and survival, and regulates electrolyte homeostasis. WNK1 belongs to the Ser/Thr protein kinase family of the protein kinase superfamily, and contains one protein kinase domain. Existing as five alternatively spliced isoforms, WNK1 is widely expressed but is found at highest levels in skeletal muscke, heart, testis and kidney. The gene that encodes WNK1 maps to human chromosome 12p13.33, and when defective, is the cause of an an autosomal dominant disease known as pseudohypoaldosteronism type II (PHAII), as well as a hereditary sensory and autonomic neuropathy designated hereditary sensory and autonomic neuropathy type 2A (HSAN2A). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of WNK1 of mouse and human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of WNK1 of mouse and human origin by WB, IP and ELISA |
| Entrez Gene | 65125 |
|---|
Application
Western blot analysis of WNK1 expression in T-47D (A), A-375 (B) and HeLa (C) whole cell lysates and mouse kidney tissue extract (D).| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
