Application
Western blot analysis of PP2B-Aγ expression in A-375 whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue showing cytoplasmic localization.| Product Name | PP2B-Aγ Antibody (S-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of PP2B-Aγ of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunit have been identified, designated PP1, PP2A, PP2B and PP2C. An additional protein phosphatase catalytic subunit, PPX (also known as PP4), is a putative member of a novel PP family. The PP2B family comprises subfamily members PP2B-Aå, PP2B-A∫ and PP2B-A©. Two additional regulatory subunits been identified, designated PP2B-B1 and PP2B-B2.87292 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of PP2B-A&gamma of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of PP2B-Aγ of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 5533 |
|---|
Application
Western blot analysis of PP2B-Aγ expression in A-375 whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue showing cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
