Application
Western blot analysis of PEMT expression in non-transfected: (A) and human PEMT transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PEMT expression in T-47D whole cell lysate.| Product Name | PEMT Antibody (P-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of PEMT of human origin by WB, IP and ELISA |
| Modification Notes | Phosphatidylethanolamine-N-methyltransferase (PEMT) catalyzes the conversion of phosphatidylethanolamine to phosphatidylcholine (PC) through three sequential methylation reactions. This pathway is primarily utilized in liver, whereas other cells utilize the 1,2-diacylglycerol cholinephosphotransferase (CDP-choline) pathway. PEMT activity participates in many physiologic processes, including the flux of lipid between liver and plasma and the delivery of essential fatty acids to blood and peripheral tissues via liver-derived lipoproteins. PEMT2, an isoform of the enzyme, regulates hepatocyte cell division by inhibiting proliferation. Loss of PEMT2 may contribute to the onset of liver carcinogenesis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of PEMT of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of PEMT of human origin by WB, IP and ELISA |
| Entrez Gene | 10400 |
|---|
Application
Western blot analysis of PEMT expression in non-transfected: (A) and human PEMT transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PEMT expression in T-47D whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
