Application
Western blot analysis of 5-LO phosphorylation in untreated (A) and lambda protein phosphatase-treated (B) rat prefrontal cortex tissue extracts.| Product Name | p-5-LO Antibody (Ser 523) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Ser 523 phosphorylated 5-LO of h and r origin by WB and IP |
| Modification | Phosphoralated |
| Modification Notes | 5-lipoxygenase (5-LO) is expressed primarily in polymorphonuclear leukocytes, macrophages, and mast cells (1). 5-LO performs the first two catalytic reactions in the biosynthesis of leukotrienes, lipid metabolites that induce contractions of airway smooth muscle and increase vascular permeability during anaphylaxis (1,2). The cellular localization of 5-LO varies between cell types (3). In activated blood polymorphonuclear leukocytes 5-LO undergoes calcium dependent translocation from the cytosol to the nuclear envelope (4). In alveolar macrophages, the majority of 5-LO is localized in the nucleus and, upon activation of these cells, intranuclear 5-LO binds to the nuclear membrane (5). This intracellular shuttling of 5-LO is dependent on the association with various signaling molecules, phosphorylation and the presence of a distinct nuclear localization signal, which is encoded at the amino terminus of 5-LO (6,7). Human and rat 5-LO are phosphorylated on Ser 523 by PKA which has an inhibitory effect on 5-LO. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 523 phosphorylated 5-LO of h and r origin by WB and IP |
|---|---|
| Tissue Specificity | recommended for detection of Ser 523 phosphorylated 5-LO of h and r origin by WB and IP |
| Entrez Gene | 240 |
|---|
Application
Western blot analysis of 5-LO phosphorylation in untreated (A) and lambda protein phosphatase-treated (B) rat prefrontal cortex tissue extracts.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
