Application
Western blot analysis of NF-1A expression in Jurkat whole cell lysate.| Product Name | NF-1A Antibody (K-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of NF-1A of mouse, rat and human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Modification Notes | The NF-1 family of CCAAT box binding proteins function to stimulate DNA replication and activate transcription. NF-1A (nuclear factor I/A), a member of the NF-1 family, is a 509 amino acid protein that localizes to the nucleus and contains one CTF/NF-I DNA-binding domain. Existing as a homodimer that is able to bind DNA, NF-1A recognizes and binds to the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' (a sequence that is common in both cellular and viral promoters) and, via this binding, plays a role in transcription and replication. NF-1A is subject to DNA damage-dependent phosphorylation, probably by ATM or ATR. Multiple isoforms of NF-1A exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of NF-1A of mouse rat and human origin by WB IP and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of NF-1A of mouse, rat and human origin by WB, IP and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 4774 |
|---|
Application
Western blot analysis of NF-1A expression in Jurkat whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
