Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human spermatophore tissue showing cytoplasmic localization.| Product Name | TFIIA-α Antibody (X-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of TFIIA-α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription factors (1). Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in the formation of a multiprotein complex containing several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFIIH (1-3). Recognition of the TATA binding element by TBP, one of the first steps in transcription initiation, may be regulated by TFIIA (4,5). TFIIA consists of three subunits designated TFIIA-å, TFIIA-∫ and TFIIA-©, and it interacts with both TBP and TAF (TBP-associated factor) (6,7). It has been demonstrated that the basic region of TBP is essential for TFIIA-dependent function of TBP (8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of TFIIA-&alpha of mouse rat and human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of TFIIA-α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 2957 |
|---|
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human spermatophore tissue showing cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
