Application
Western blot analysis of SUV420H1 expression in Hep G2 whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human skin tissue showing nuclear localization.Application
Western blot analysis of SUV420H1 expression in MCF7 nuclear extract.| Product Name | SUV420H1 Antibody (Q-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of SUV420H1 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | SUV420H1 (suppressor of variegation 4-20 homolog 1), also known as CGI85 or KMT5B, is an 885 amino acid protein that is associated with pericentric heterochromatin in the nucleus. One of several members of the histone-lysine methyltransferase family, SUV420H1 functions as a histone methyltransferase that trimethylates the Lys-20 residue of Histone H4, thereby tagging H4 for transcriptional repression. Characteristic of most histone methytransferases, SUV420H1 contains one SET domain through which it confers its enzymatic activity. The co-localization of SUV420H1 with pericentric heterochromatin allows the methyltransferase to play a key role in the establishment of constitutive heterochromatin, further implicating SUV420H1 as a regulator of transcriptional events. Expression of SUV420H1 is strongly down-regulated in breast cancer cells, suggesting a role for SUV420H1 in tumor suppression. Three isoforms of SUV420H1 are expressed due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of SUV420H1 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of SUV420H1 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 51111 |
|---|
Application
Western blot analysis of SUV420H1 expression in Hep G2 whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human skin tissue showing nuclear localization.
Application
Western blot analysis of SUV420H1 expression in MCF7 nuclear extract.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
