Application
Western blot analysis of POU2F3 expression in JAR whole cell lysate (A), A-431 (B), A549 (C), Hep G2 (D) and SW480 (E) nuclear extracts and mouse placenta tissue extract (F).Application
Western blot analysis of POU2F3 expression in human placenta tissue extract.| Product Name | POU2F3 Antibody (J-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of POU2F3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Modification Notes | Tissue-restricted POU domain transcription factors, which bind octamer or octamer-like gene sequences, play roles in cellular differentiation and the development of several organs. POU2F3, also designated Oct-11, PLA-1 protein or transcription factor Skn-1, represents a member of the class 2 POU domain family of transcriptional activators, highly related to Oct-2, that are selectively expressed in terminally differentiating epidermal and hair follicles. POU2F3 is characterized by an N-terminal domain that inhibits DNA binding and can inhibit transactivation by Oct-2. Alternative splicing of the N-terminus serves to activate cytokeratin 10 (K10) gene expression. When POU2F3 is expressed in eukaryotic cells it can bind to an octamer site, suggesting that in vivo cellular factors modulate the activity of the inhibitory domain to permit DNA-binding. The inhibitory domain does not allow transactivation by POU2F3 or by a heterologous transactivator containing this domain in cis. POU2F3 contributes to epidermal stratification by primarily promoting keratinocyte proliferation and secondarily by enhancing the subsequent keratinocyte differentiation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of POU2F3 of mouse rat and human origin by WB IP IF IHC(P) and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of POU2F3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 25833 |
|---|
Application
Western blot analysis of POU2F3 expression in JAR whole cell lysate (A), A-431 (B), A549 (C), Hep G2 (D) and SW480 (E) nuclear extracts and mouse placenta tissue extract (F).
Application
Western blot analysis of POU2F3 expression in human placenta tissue extract.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
