Application
Western blot analysis of human iASPP transfected 293T whole cell lysate.| Product Name | iASPP Antibody (E-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of iASPP of human origin by WB, IP and ELISA |
| Modification Notes | Apoptosis stimulating protein of p53 (ASPP) is a family of proteins that act as regulators of apoptosis via their interactions with p53. ASPP1 and ASPP2 are both members of the ASPP family that regulate p53 by enhancing its transactivation function and binding to proapoptotic genes. iASPP, is the third member of the ASPP family and is considered inhibitory as it negatively regulates p53. iASPP is the most evolutionarily conserved inhibitor of p53 induced apoptosis. Expression of iASPP is upregulated in human breast carcinomas that express wildtype p53. Overexpression of iASPP may play a role in leukemogenesis and progression of acute leukemia. Inhibiting iASPP may be an effective strategy for treating tumors expressing wildtype p53. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of iASPP of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of iASPP of human origin by WB, IP and ELISA |
| Entrez Gene | 10848 |
|---|
Application
Western blot analysis of human iASPP transfected 293T whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
