Application
Antibodies tested include p-MEK-1 (Thr 386): (A,B,C) and MEK-1 (H-8): (D,E,F).Application
Western blot analysis of MEK-1 phosphorylation in untreated (A) and lambda protein phosphatase-treated (B) T-47D whole cell lysates.| Product Name | p-MEK-1 Antibody (Thr 386) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Thr 386 phosphorylated MEK-1 of mouse, rat, human and Xenopus origin by WB and IP; also reactive with additional species, including bovine and canine |
| Modification | Phosphoralated |
| Modification Notes | A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK (1). A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity (2). MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases (3). MEK-5 appears to specifically phosphorylate ERK5 (4), whereas MEK-6 phosphorylates p38 and p38ß (5,6). MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway (7–9). The phosphorylation of various serine and threonine residues, such as Thr 386, activates MEK-1 function. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Thr 386 phosphorylated MEK-1 of mouse rat human and Xenopus origin by WB and IP also reactive with additional species including bovine and canine |
|---|---|
| Tissue Specificity | recommended for detection of Thr 386 phosphorylated MEK-1 of mouse, rat, human and Xenopus origin by WB and IP; also reactive with additional species, including bovine and canine |
| Entrez Gene | 5604 |
|---|
Application
Antibodies tested include p-MEK-1 (Thr 386): (A,B,C) and MEK-1 (H-8): (D,E,F).
Application
Western blot analysis of MEK-1 phosphorylation in untreated (A) and lambda protein phosphatase-treated (B) T-47D whole cell lysates.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
