Application
Western blot analysis of Synapsin Ia/b phosphorylation in untreated (A) and lambda protein phosphatase-treated (B) rat prefrontal cortex tissue extracts.| Product Name | p-Synapsin Ia/b Antibody (Ser 603) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Ser 603 phosphorylated Synapsin Ia/b of rat origin and correspondingly Ser 605 phosphorylated Synapsin Ia/b of mouse and human origin by WB and IP; also reactive with additional species, including bovine, Xenopus and zebrafish; |
| Modification | Phosphoralated |
| Modification Notes | Synapsin I, which exists as two alternatively spliced isoforms designated Synapsin Ia and Synapsin Ib, has been characterized as one of the major phosphoproteins in nerve terminals and is thought to be involved in the regulation of neurotransmitter release. Synapsin I cross-links synaptic vesicles and the cytoskeleton, and the interactions of synapsins with actin filaments and synaptic vesicles are regulated by phosphorylation by calmodulin-dependent protein kinase II and cAMP-dependent protein kinase. Posttranslational modifications of Synapsin I result in phosphorylation of the protein at different sites and by different kinases. The Ser 553 residue of Synapsin I is phosphorylated in vivo. This phosphorylation site is immediately followed by a proline, suggesting that Synapsin I is an in vivo substrate of the proline-directed protein kinase, Cdk5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Ser 603 phosphorylated Synapsin Ia/b of rat origin and correspondingly Ser 605 phosphorylated Synapsin Ia/b of mouse and human origin by WB and IP also reactive with additional species including bovine Xenopus and zebrafish |
|---|---|
| Tissue Specificity | recommended for detection of Ser 603 phosphorylated Synapsin Ia/b of rat origin and correspondingly Ser 605 phosphorylated Synapsin Ia/b of mouse and human origin by WB and IP; also reactive with additional species, including bovine, Xenopus and zebrafish; also reactive with additional species, including bovine |
| Entrez Gene | 6853 |
|---|
Application
Western blot analysis of Synapsin Ia/b phosphorylation in untreated (A) and lambda protein phosphatase-treated (B) rat prefrontal cortex tissue extracts.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
