Application
Western blot analysis of MPDU1 expression in Hep G2 whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Product Name | MPDU1 Antibody (I-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of MPDU1 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | MPDU1 (Mannose-P-dolichol utilization defect 1 protein), also designated suppressor of Lec15 and Lec35 glycosylation mutation or SL15, mediates the transfer of glucose and mannose residues from Glc-P-Dol and Man-P-Dol to oligosaccharides. Defects in the MPDU1 gene result in a Type I congenital disorder of glycosylation CDG-If. Patients with CDG-If make incomplete lipid-linked oligosaccharides (LLO) and present with severe psychomotor retardation, seizures, failure to thrive, dry skin and scaling with erythroderma and impaired vision. Overexpression of GlcNAc-1-P transferase has been shown to impair the function of MPDU1, suggesting a form of pseudo-CDG-If. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of MPDU1 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of MPDU1 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 9526 |
|---|
Application
Western blot analysis of MPDU1 expression in Hep G2 whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
