Application
Western blot analysis of phosphorylated Stat1 expression in untreated (A) and EGF-treated (B) MCF7 cell extracts.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear and cytoplasmic localization.| Product Name | p-Stat1 Antibody (Tyr 701) |
|---|---|
| Antibody Type | Primary Antibodies |
| Conjugation | Agarose |
| Modification | Phosphoralated |
| Modification Notes | Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-a and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3∫ appears to be activated by both while Stat3å is activated by EGF, but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways. Mouse, rat and human Stat1 are phosphorylated on tyrosine and serine residues, specifically Tyr 701 which is phosphorylated by JAK1, which promotes dimerization, allowing Stat1 to be translocated to the nucleus. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFIHCIPWB |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:100~1:500 IHC:1:50~1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Tissue Specificity | recommended for detection of Tyr 701 phosphorylated Stat1 of mouse, rat and human origin by WB, IP, IF and IHC(P) |
|---|
| Entrez Gene | 6772 |
|---|
Application
Western blot analysis of phosphorylated Stat1 expression in untreated (A) and EGF-treated (B) MCF7 cell extracts.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear and cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:100~1:500 IHC:1:50~1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
