Application
Blots were probed with p-MARCKS (Ser 159): 101730 preincubated with its cognate phosphorylated peptide (A) and p-MARCKS (Ser 159): 101730 (B).| Product Name | p-MARCKS Antibody (Ser 159) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Ser 159 of MARCKS of human origin |
| Modification | Phosphoralated |
| Modification Notes | Myristolated Alanine-Rich C-Kinase Substrate (MARCKS) is a major in vivo substrate of protein kinase C (PKC) and for the lipid-activated PKC-related kinase (PRK1) (1). Furthermore, PRK1 and PKC phosphorylate MARCKS on the same sites in vitro, serine 152, 156 and 163. MARCKS serves as an in vitro substrate for PKC m as well. However, in contrast to other PKCs, MARCKS is phosphorylated by PKC m only at serine 156 and not at serines 152 and 163, implicating a differential regulation by PKC m. Therefore, control of MARCKS phosphorylation on these previously identified 'PKC' sites may be regulated under certain circumstances by PRK as well as PKC mediated signalling pathways. MARCKS associates with the plasma membrane in response to PKC-catalyzed phosphorylation of MARCKS. It has been suggested that MARCKS is capable of associating with the plasma membrane through binding to phospholipids without interaction with membranous proteins. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| recommended for detection of Ser 159/ phosphorylated MARCKS of mouse, rat and human origin by WB and IP: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 159 of MARCKS of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 159 of MARCKS of human origin |
| Entrez Gene | 4082 |
|---|
Application
Blots were probed with p-MARCKS (Ser 159): 101730 preincubated with its cognate phosphorylated peptide (A) and p-MARCKS (Ser 159): 101730 (B).| Application Notes | recommended for detection of Ser 159/ phosphorylated MARCKS of mouse, rat and human origin by WB and IP: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
