Application
Western blot analysis of RUFY1 expression in HeLa whole cell lysate.| Product Name | RUFY1 Antibody (H-125) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 461-585 mapping within an internal region of RUFY1 of human origin |
| Modification Notes | RUFY1 (RUN and FYVE domain containing 1), also known as RABIP4 or ZFYVE12, is a 708 amino acid protein that localizes to the cytoplasm and the early endosome membrane. Highly expressed in testis, lung, brain and kidney, RUFY1 functions to bind phosphatidylinositol 3-phosphate-containing phospholipid vesicles and, via this interaction, participates in early endosomal trafficking. RUFY1 contains one RUN domain and one FYVE-type zinc finger through which it mediates its ability to bind phosphatidylinositol 3-phosphate. Upon DNA damage, RUFY1 may be phosphorylated by ATM or ATR. Additionally, the phosphorylation of Tyr-389 and/or Tyr-400 residues on human RUFY1 is thought to be necessary for endosomal localization. Three isoforms of RUFY1 exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of RUFY1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 461-585 mapping within an internal region of RUFY1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 461-585 mapping within an internal region of RUFY1 of human origin |
| Entrez Gene | 80230 |
|---|
Application
Western blot analysis of RUFY1 expression in HeLa whole cell lysate.| Application Notes | recommended for detection of RUFY1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
