Application
Western blot analysis of TK2 expression in mouse muscle tissue extract (A) and Hep G2 whole cell lysate (B).| Product Name | TK2 Antibody (B-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of TK2 of mouse and human origin by WB, IP and ELISA |
| Modification Notes | Thymidine kinase 2 (TK2) salvages mitochondrial (mt) pyrimidine deoxynucleosides for mtDNA precursor synthesis. TK2 phosphorylates these nucleosides to their corresponding nucleoside monophosphates using a nucleotide triphosphate as a donor. Deficiency of mitochondrial TK2 manifests as severe skeletal myopathy during infancy, due to depletion of mtDNA. Mutant enzyme possesses similar K(m) values to wild type, however, the V(max) is markedly decreased, leading to the decreased enzyme efficiency, which causes the disease. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of TK2 of mouse and human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of TK2 of mouse and human origin by WB, IP and ELISA |
| Entrez Gene | 7084 |
|---|
Application
Western blot analysis of TK2 expression in mouse muscle tissue extract (A) and Hep G2 whole cell lysate (B).| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
