Application
Cytosolic labeling of surface epithelial cells in SDS-denatured mouse colon tissue sections with the primary antibody diluted to 1:350. Green label = MPST, red label = UAE1 lectin.Kindly provided by Christian Lytle, Ph.D. University of California, Riverside.Application
Western blot analysis of MPST expression in non-transfected 293T: (A), human MPST transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of MPST expression in non-transfected: (A) and mouse MPST transfected: (B) 293T whole cell lysates.| Product Name | MPST Antibody (L-13) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | MPST (mercaptopyruvate sulfurtransferase), also known as MST or TST2, is a 297 amino acid protein that localizes to the cytoplasm and contains two rhodanese domains. Existing as a monomer or as a dilsulfide-linked homodimer, MPST functions to catalyze the transfer of a sulfur ion to select thiol compounds, such as cyanide, and is thought to be involved in cyanide detoxification and cysteine degradation. MPST deficiency may be associated with the pathogenesis of the rare disorder mercaptolactate-cysteine disulfiduria (MCDU). The gene encoding MPST maps to human chromosome 22, which houses over 500 genes and is the second smallest human chromosome. Mutations in several of the genes that map to chromosome 22 are involved in the development of Phelan-McDermid syndrome, Neurofibromatosis type 2, autism and schizophrenia. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MPST of mouse, rat and human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of MPST of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of MPST of human origin |
| Entrez Gene | 4357 |
|---|
Application
Cytosolic labeling of surface epithelial cells in SDS-denatured mouse colon tissue sections with the primary antibody diluted to 1:350. Green label = MPST, red label = UAE1 lectin.Kindly provided by Christian Lytle, Ph.D. University of California, Riverside.
Application
Western blot analysis of MPST expression in non-transfected 293T: (A), human MPST transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of MPST expression in non-transfected: (A) and mouse MPST transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of MPST of mouse, rat and human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
