Application
Western blot analysis of hippocalcin expression in Y79 whole cell lysate (A) and mouse brain tissue extract (B).Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human brain tissue showing cytoplasmic localization.| Product Name | hippocalcin Antibody (Z-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Hippocalcin is a neuron-specific calcium-binding protein found primarily in the plasma membrane of brain and retinal tissue, with increased expression observed in hippocampal pyramidal cells. Through its calcium-dependent signal regulation, hippocalcin can both inhibit rhodopsin kinase and increase phospholipase D2 expression. In order to regulate kinase and phospholipase activity, hippocalcin must bind to the plasma membrane where it can then bind two calcium ions for use in signal regulation. The hippocalcin protein is highly conserved in mouse, rat and human tissue and has a suggested role in neural plasticity and associative memory by contributing to the survival of neurons during aging. The loss of hippocalcin expression is thought to contribute to age-related impairment of post-synaptic functions related to neuronal degradation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of hippocalcin of mouse and human origin by WB IP IF IHC(P) ELISA |
|---|---|
| Tissue Specificity | recommended for detection of hippocalcin of mouse and human origin by WB, IP, IF, IHC(P), ELISA |
| Entrez Gene | 3208 |
|---|
Application
Western blot analysis of hippocalcin expression in Y79 whole cell lysate (A) and mouse brain tissue extract (B).
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human brain tissue showing cytoplasmic localization.| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
