Application
Western blot analysis of HUGT2 expression in MIA PaCa-2 (A), C2C12 (B), A-10 (C) and HeLa (D) whole cell lysates.| Product Name | HUGT2 Antibody (N-16) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | HUGT2 is also known as UGCGL2 (UDP-glucose ceramide glucosyltransferase-like 2) or UGT2 (UDP-glucose: glycoprotein glucosyltransferase 2) and is a 1,516 amino acid protein that is expressed abundantly in kidney, pancreas, heart and skeletal muscle. HUGT2 is localized to the lumen of the ER (endoplasmic reticulum) and to the ER-Golgi intermediate compartment which regulates transportation between the ER and the Golgi. HUGT2 is a quality control protein that targets unfolded glycoproteins for glucosylation in a calcium-dependent manner, but may use manganese is the absence of calcium. HUGT2 targets glycans by attaching single glucose residues to glycans with amino acid sequences which are not correctly folded, an enzymatic process that is exhibited during carbohydrate processing. Patients with type 2 diabetes express altered HUGT2 proteins in their beta cells, suggesting that these patients may also have defects in their ER glycoprotein quality control abilities. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of HUGT2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the N-terminus of HUGT2 of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the N-terminus of HUGT2 of human origin |
| Entrez Gene | 55757 |
|---|
Application
Western blot analysis of HUGT2 expression in MIA PaCa-2 (A), C2C12 (B), A-10 (C) and HeLa (D) whole cell lysates.| Application Notes | recommended for detection of HUGT2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
