Application
Western blot analysis of UBE2C expression in non-transfected: (A) and human UBE2C transfected: (B) 293 whole cell lysates.Application
Western blot analysis of UBE2C expression in non-transfected: (A) and human UBE2C transfected: (B) 293 whole cell lysates.| Product Name | UBE2C Antibody (H-90) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires the ATP-dependent activation of the Ub C-terminus and the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. UBE2C, also designated UBCH10 in human, is an E2 ubiquitin conjugating enzyme for the anaphase promoting complex (APC), which coordinates mitosis and G1 by sequentially promoting the degradation of key cell-cycle regulators. UBE2C is overexpressed in many different types of cancers and may be a potential therapeutic target. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of UBE2C of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 17-106 mapping within an internal region of UBE2C of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 17-106 mapping within an internal region of UBE2C of human origin |
| Entrez Gene | 11065 |
|---|
Application
Western blot analysis of UBE2C expression in non-transfected: (A) and human UBE2C transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of UBE2C expression in non-transfected: (A) and human UBE2C transfected: (B) 293 whole cell lysates.| Application Notes | recommended for detection of UBE2C of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
