Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of MAD1 expression in non-transfected 293T: (A), human MAD1 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of MAD1 expression in non-transfected 293T: (A), mouse MAD1 transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.Application
Western blot analysis of MAD1 expression in non-transfected 293T: (A), human MAD1 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of MAD1 expression in A-431 (A) and HeLA (B) whole cell lysates and HeLa nuclear extract (C).| Product Name | MAD1 Antibody (H-228) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Cell cycle progression is subject to arrest at the mitotic spindle assembly checkpoint in response to incorrect spindle fiber assembly. MAD1 and MAD2 (for mitotic arrest-deficient 1 and 2) are components of the mitotic spindle checkpoint. Incorrect spindle assembly in normal cells leads to mitotic arrest. MAD1 prevents the onset of anaphase until all chromosomes are aligned correctly at the metaphase plate and is crucial for anchoring MAD2L1 to the nuclear periphery. It also plays an important role in septum positioning. MAD1 can form a homo-dimer, but may also form a heterodimer with MAD2 to form the tetrameric MAD1L1-MAD2L1 core complex. MAD1 localizes primarily to the nucleus, but during mitosis, it moves from a nuclear distribution to the centrosome, to the spindle midzone and then on to the midbody. MAD1 activity is induced by BUB1 and the protein is hyperphosphorylated after mitotic spindle damage and/or in late S through M phase. Defects in the gene encoding for MAD1, MAD1L1, play a major role in the development and progression of various cancer types. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MAD1 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 491-718 mapping at the C-terminus of MAD1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 491-718 mapping at the C-terminus of MAD1 of human origin |
| Entrez Gene | 8379 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of MAD1 expression in non-transfected 293T: (A), human MAD1 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of MAD1 expression in non-transfected 293T: (A), mouse MAD1 transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.
Application
Western blot analysis of MAD1 expression in non-transfected 293T: (A), human MAD1 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of MAD1 expression in A-431 (A) and HeLA (B) whole cell lysates and HeLa nuclear extract (C).| Application Notes | recommended for detection of MAD1 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
