Application
Western blot analysis of CLIC5 expression in non-transfected: (A) and human CLIC5 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CLIC5 expression in non-transfected 293T: (A), human CLIC5 transfected 293T: (B) and JAR (C) whole cell lysates.Application
Western blot analysis of CLIC5 expression in Jurkat whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing cytoplasmic localization.| Product Name | CLIC5 Antibody (B-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of CLIC5 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | Chloride intracellular channel 5 (CLIC5), is a member of the highly conserved family of chloride ion channels that function in both soluble and integral membrane forms. Chloride channels regulate cellular traffic of chloride ions, a critical component of all living cells. They are involved in membrane potential stabilization, signal transduction, cell volume regulation and organic solute transport. CLIC5 associates with actin-based cytoskeletal structures and may be involved in their assembly and maintenance. In addition, CLIC5 may also play an important role in inner ear function localizing to the stereocilia and possibly associating with Radixin. Two CLIC5 isoforms exist and they are referred to as CLIC5A and CLIC5B. The two isoforms share an identical C-terminus but have distinct N-terminal sequences. CLIC5B is the human ortholog of bovine p64 and avian p62. It binds AKAP350 and localizes to the Golgi apparatus. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of CLIC5 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of CLIC5 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 53405 |
|---|
Application
Western blot analysis of CLIC5 expression in non-transfected: (A) and human CLIC5 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CLIC5 expression in non-transfected 293T: (A), human CLIC5 transfected 293T: (B) and JAR (C) whole cell lysates.
Application
Western blot analysis of CLIC5 expression in Jurkat whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney tissue showing cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
