Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic and nuclear staining of cells in seminiferous ducts and Leydig cells.Application
Western blot analysis of Endo180 expression in non-transfected: (A) and human Endo180 transfected: (B) 293T whole cell lysates.| Product Name | Endo180 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Endo180 (Endocytic receptor 180), also known as macrophage mannose receptor C type 2 (MRC2), urokinase plasminogen activator receptor-associated protein (uPARAP) or CD280 antigen, is a ubiquitously expressed, type I membrane protein with predominant expression in fetal lung and kidney. The extracellular domain of Endo180 contains a fibronectin type II domain, eight C-type lectin domains and a cysteine-rich domain. Endo180 functions as a cell surface receptor and mediates collagen matrix remodeling as well as cell migration by playing a role in the uptake and lysosomal degradation of collagen. Endo180 can bind to gelatin and collagens I, II, IV and V. It may play a role in wound regeneration. In addition, Endo180 can form a complex with uPAR and pro-uPA, thereby partaking in a variety of cellular proteolytic and signaling functions. Endo180 participates in the destruction of connective tissue during the progression of head and neck squamous cell carcinoma. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of endocytic receptor 180 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1051-1350 mapping within an extracellular domain of Endo180 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1051-1350 mapping within an extracellular domain of Endo180 of human origin |
| Entrez Gene | 9902 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing cytoplasmic and nuclear staining of cells in seminiferous ducts and Leydig cells.
Application
Western blot analysis of Endo180 expression in non-transfected: (A) and human Endo180 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of endocytic receptor 180 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
