Application
Western blot analysis of PNPase expression in non-transfected 293T: (A), human PNPase transfected 293T: (B) and SK-MEL-28 (C) whole cell lysates.| Product Name | PNPase Antibody (H-124) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Mitochondrial polyribonucleotide nucleotidyltransferase, also designated 3'-5' RNA exonuclease, OLD35, PNPase or PNPT1, is an evolutionaly conserved protein in which the mouse protein shares 90% identity with the human version. PNPase (polyribonucleotide nucleotidyltransferase 1) participates in mRNA degradation and hydrolyzes single-stranded ribonucleotides in the 3' to 5' direction. PNPase forms homotrimers and is upregulated in response to interferon-b induction. The N-terminus of PNPase contains a putative mitochondrial targeting sequence; mutation analysis confirms that N-terminal sequences of PNPase target the protein to the mitochondria. Endogenous PNPase also co-localizes with a mitochondrial marker protein in HeLa cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PNPase of mouse, rat and human origin by WB, IP, IF and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 656-779 mapping near the C-terminus of PNPase of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 656-779 mapping near the C-terminus of PNPase of human origin |
| Entrez Gene | 87178 |
|---|
Application
Western blot analysis of PNPase expression in non-transfected 293T: (A), human PNPase transfected 293T: (B) and SK-MEL-28 (C) whole cell lysates.| Application Notes | recommended for detection of PNPase of mouse, rat and human origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
