Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human liver tissue showing nuclear and cytoplasmic localization.Application
Western blot analysis of ZNF654 expression in 293T whole cell lysate.| Product Name | ZNF654 Antibody (D-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Krüppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. As a member of the krueppel C2H2-type zinc-finger protein family, ZNF654 (Zinc finger protein 654), also known as Melanoma-associated antigen, is a 581 amino acid nuclear protein that contains five C2H2-type zinc fingers. The gene encoding ZNF654 maps to human chromosome 3, which is made up of about 214 million bases encoding over 1,100 genes, including a chemokine receptor (CKR) gene cluster and a variety of human cancer-related gene loci. Both ZNF654 and Vgl-3 are underexpressed in malignant ovarian tumor samples, suggesting that these proteins may play a role in ovarian tumorigenesis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of ZNF654 of mouse rat and human origin by WB IP IF IHC(P) and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of ZNF654 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 55279 |
|---|
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human liver tissue showing nuclear and cytoplasmic localization.
Application
Western blot analysis of ZNF654 expression in 293T whole cell lysate.| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
