Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human nasopharynx tissue showing cytoplasmic and membrane staining of glandular cells.Application
Western blot analysis of Cytokeratin 19 expression in MCF7 whole cell lysate.| Product Name | Cytokeratin 19 Antibody (H-60) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells and have been found to be useful markers of tissue differentiation, which is directly applicable to the characterization of malignant tumors. For example, many types of cancer cells express Cytokeratin 19 (CK19), an epithelial cytoskeletal protein within the suprabasal squamous epithelium. Cytokeratin 19 is a specific marker of moderate to severe dysplasia and carcinoma in situ in oral cavity squamous epithelium, and measurement of Cytokeratin 19 may be a useful marker in diagnosing hepatoma. Cytokeratin 19 fragment levels in serum have been documented as a marker for lung cancer. Clinical investigations have suggested that serum CYFRA 21-1, a fragment of Cytokeratin 19, may be among the most useful tumor markers. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Cytokeratin 19 of mouse, human and, to a lesser extent, rat origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-80 mapping near the N-terminus of Cytokeratin 19 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-80 mapping near the N-terminus of Cytokeratin 19 of human origin |
| Entrez Gene | 3880 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human nasopharynx tissue showing cytoplasmic and membrane staining of glandular cells.
Application
Western blot analysis of Cytokeratin 19 expression in MCF7 whole cell lysate.| Application Notes | recommended for detection of Cytokeratin 19 of mouse, human and, to a lesser extent, rat origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
