| Product Name | RasGRP3 Antibody (H-70) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Members of the Ras subfamily of GTPases function in signal transduction as GTP/GDP-modulated switches that rotate between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RasGRP3 (GRP3), act as Ras activators by promoting retrieval of GTP to maintain the active GTP-bound state and are the fundamental link between cell surface receptors and Ras activation. Highest levels of RasGRP3 expression are observed in heart, brain, lung and kidney tissues, and intermediate expression is observed in liver, skeletal muscle, pancreas, spleen, testis and ovary tissues. RasGRP3, which shares significant sequence identity with the calcium- and diacylglycerol-activated GEFs, activates Ras and Rap 1 and promotes activation of ELK1 in prostate cancer cell lines. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of RasGRP3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-69 mapping at the N-terminus of RasGRP3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-69 mapping at the N-terminus of RasGRP3 of human origin |
| Entrez Gene | 25780 |
|---|
| Application Notes | recommended for detection of RasGRP3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
