Application
Western blot analysis of LITAF expression in non-transfected 293T: (A), mouse LITAF transfected 293T: (B), human PBL (C), rat PBL (D) and A-431 (E) whole cell lysates.Application
Western blot analysis of LITAF expression in RAW 264.7 whole cell lysate.Application
Western blot analysis of LITAF expression in non-transfected 293T: (A), human LITAF transfected 293T: (B) and HL-60 (C) whole cell lysates and mouse brain tissue extract (D).Application
Western blot analysis of LITAF expression in non-transfected 293: (A), mouse LITAF transfected 293: (B), HeLa (C) and Hep G2 (D) whole cell lysates.| Product Name | LITAF Antibody (FL-161) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Lipopolysaccharide (LPS) is a potent stimulator of monocytes and macrophages, causing secretion of tumor necrosis factor alpha (TNFå) and other inflammatory mediators. The inflammatory response to bacteria and bacterial products, such as LPS, is mediated by a variety of secreted factors, but cytotoxic effects of LPS have been ascribed to TNFå activity. LITAF (LPS-induced TNFå factor), Stat6B and the LITAF-Stat6B complex all play a role in the regulation of inflammatory cytokines in response to LPS or p53 stimulation in mammalian cells. LITAF is a nuclear protein crucial in TNFå gene transcription regulation. High levels of expression of LITAF mRNA have been observed predominantly in the placenta, peripheral blood leukocytes, lymph nodes and spleen. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of LITAF of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-161 representing full length LITAF of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-161 representing full length LITAF of human origin |
| Entrez Gene | 9516 |
|---|
Application
Western blot analysis of LITAF expression in non-transfected 293T: (A), mouse LITAF transfected 293T: (B), human PBL (C), rat PBL (D) and A-431 (E) whole cell lysates.
Application
Western blot analysis of LITAF expression in RAW 264.7 whole cell lysate.
Application
Western blot analysis of LITAF expression in non-transfected 293T: (A), human LITAF transfected 293T: (B) and HL-60 (C) whole cell lysates and mouse brain tissue extract (D).
Application
Western blot analysis of LITAF expression in non-transfected 293: (A), mouse LITAF transfected 293: (B), HeLa (C) and Hep G2 (D) whole cell lysates.| Application Notes | recommended for detection of LITAF of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
