Application
Western blot analysis of DAGLα expression in mouse skeletal muscle tissue extract.| Product Name | DAGLα Antibody (C-14) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Members of the AB hydrolase superfamily have diverse catalytic functions and play a crucial role in the metabolism of lipids. DAGLå (diacylglycerol lipase alpha), also known as NSDDR or C11orf11, is a 1,042 amino acid multi-pass membrane protein that belongs to the AB hydrolase superfamily. Highly expressed in brain and pancreas, DAGLå uses calcium as a cofactor to catalyze the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), a reaction that is required for axonal growth and for retrograde synaptic signaling at mature synapses. DAGLå functions as at optimal pH of 7 and its activity is inhibited by p-hydroxy-mercuri-benzoate and HgCl2. The gene encoding DAGLå maps to human chromosome 11, which houses over 1,400 genes and comprises nearly 4% of the human genome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of DAGL&alpha isoforms 1-3 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with DAGL&beta also reactive with additional species, including equine, canine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within a C-terminal cytoplasmic domain of DAGL&alpha of human origin |
|---|---|
| Tissue Specificity | epitope mapping within a C-terminal cytoplasmic domain of DAGLα of human origin |
| Entrez Gene | 747 |
|---|
Application
Western blot analysis of DAGLα expression in mouse skeletal muscle tissue extract.| Application Notes | recommended for detection of DAGL&alpha isoforms 1-3 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with DAGL&beta also reactive with additional species, including equine, canine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
