Application
Western blot analysis of synphilin-1 expression in IMR-32 whole cell lysate (A) and mouse brain tissue extract (B).| Product Name | synphilin-1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Synphilin-1 (alpha synuclein interacting protein,SNCAIP) is a 919-amino acid protein that associates with alpha synuclein and promotes the formation of cytosolic inclusions in neuronal cells (1,2). The synuclein family members, including alpha-synuclein and beta-synuclein, are predominantly expressed in the brain where they influence synaptic regulation and neuronal plasticity (3,4).Synphilin-1 contains modular protein domains, such as ankyrin-like repeats and a coiled-coil domain (5). While both alpha-synuclein and synphilin-1 are co-expressed in Lewy bodies of patients with Parkinson's disease (PD), only mutations in the gene for alpha-synuclein have been determined to confer pathogenicity (6,7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of synphilin-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of synphilin-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of synphilin-1 of human origin |
| Entrez Gene | 9627 |
|---|
Application
Western blot analysis of synphilin-1 expression in IMR-32 whole cell lysate (A) and mouse brain tissue extract (B).| Application Notes | recommended for detection of synphilin-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
