Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing nucleolar, nuclear and cytoplasmic staining of glandular cells.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nucleolar and nuclear localization.Application
Western blot analysis of NDH II expression in HeLa nuclear extract.| Product Name | NDH II Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Pre-mRNA splicing is a critical step in the posttranscriptional regulation of gene expression. Several protein complexes are involved in proper mRNA splicing and transport. The small nuclear ribonucleoprotein particles (snRNPs) interact with the SRm160/300 splicing coactivator complex to form a large RNA spliceosome. The heterogeneous nuclear ribonucleoproteins (hnRNPs) contribute to the processing and transport of pre-mRNA within the spliceosome. Also, the exon junction complex (EJC), which includes Y14, Aly/REF and Magoh, mediates mRNA export, cytoplasmic localization and nonsense-mediated mRNA decay. The effect on pre-mRNA splicing involves a nuclear complex (CBC). CBC consists of two cap binding proteins, CBP20 and CBP80, which mediate the stimulatory functions of the cap in pre-mRNA splicing, 3'-end formation and U snRNA export. Splicing factor 1 is a nuclear protein that binds the branch point sequence of pre-mRNA in the first step of spliceosome assembly and SRp55 modulates the selection of alternative splice sites in constitutive splicing. Nuclear DNA helicase II (NDH II), also known as RNA helicase A, generates secondary structures that interact with RNA-binding proteins. MDA5 is an ATP-dependent RNA helicase associated with the growth, differentiation and death of human melanoma cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of nuclear DNA helicase II of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of NDH II of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of NDH II of human origin |
| Entrez Gene | 1660 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing nucleolar, nuclear and cytoplasmic staining of glandular cells.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nucleolar and nuclear localization.
Application
Western blot analysis of NDH II expression in HeLa nuclear extract.| Application Notes | recommended for detection of nuclear DNA helicase II of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
