Application
Western blot analysis of GCNF expression in F9 whole cell lysate (A) and human recombinant GCNF fusion protein (B).| Product Name | GCNF Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Germ cell nuclear factor (GCNF) is an orphan member of the nuclear receptor gene superfamily that influences neurogenesis and germ cell development. GCNF can homodimerize and bind DNA. GCNF regulates paracrine interaction between the oocyte and somatic cells by regulating the expression of BMP-15 and GDF-9, to affect female fertility. GCNF is present in spermatocytes and round spermatids of adult male mouse testis; northern blot and ribonuclease protection assays have shown GCNF is predominant in the testis. The gene expresses three alternatively spliced transcript variants. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of GCNF of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 181-480 mapping at the C-terminus of GCNF of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 181-480 mapping at the C-terminus of GCNF of human origin |
| Entrez Gene | 2649 |
|---|
Application
Western blot analysis of GCNF expression in F9 whole cell lysate (A) and human recombinant GCNF fusion protein (B).| Application Notes | recommended for detection of GCNF of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
