Application
Western blot analysis of Rac GAP1 expression in Jurkat (A) and K-562 (B) whole cell lysates.| Product Name | Rac GAP1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | A large number of low molecular weight, GTP binding proteins of the Ras superfamily have been identified. These proteins regulate many fundamental processes in all eukaryotic cells such as growth, vesicle traffic and cytoskeletal organization. GTPase-activating proteins (GAPs) accelerate the intrinsic rate of GTP hydrolysis of Ras-related proteins, resulting in downregulation of their active form. Through this function, GAPs negatively regulate Ras-mediated signaling. Rac GAP1 (Rac GTPase activating protein 1), also known as MgcRacGAP (male germ cell Rac GTPase activating protein), ID-GAP or HsCYK-4, functions as a GAP and exhibits strong activity towards Rac 1 and Cdc42. Highly expressed in thymus, placenta and testis with lower levels in spleen and peripheral blood lymphocytes, Rac GAP1 contains one Rho-GAP domain and one phorbol-ester/DAG-type zinc finger. Rac GAP1 plays an essential role in cytokinesis, functioning as a scaffold protein as well as a GTPase regulator. During cytokinesis, Rac GAP1 is phosphorylated at multiple sites. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Rac GAP1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 142-437 mapping within an internal region of Rac GAP1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 142-437 mapping within an internal region of Rac GAP1 of human origin |
| Entrez Gene | 29127 |
|---|
Application
Western blot analysis of Rac GAP1 expression in Jurkat (A) and K-562 (B) whole cell lysates.| Application Notes | recommended for detection of Rac GAP1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
