Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing nuclear localization.Application
Western blot analysis of Med8 expression in non-transfected: (A) and mouse Med8 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Med8 expression in Hep G2 whole cell lysate.| Product Name | Med8 Antibody (FL-268) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | In mammalian cells, transcription is regulated in part by high molecular weight co-activating complexes that mediate signals between transcriptional activators and RNA polymerase II (Pol II). The mediator complex is one such multi-protein structure that functions as a bridge between regulatory proteins and Pol II, thereby regulating Pol II-dependent transcription. Med8 (mediator complex subunit 8), also known as arc32 (activator-recruited cofactor 32 kDa component), is a 268 amino acid protein that localizes to the nucleus and exists as a component of the mediator complex. Involved in the pathway of protein modification and ubiquitination, Med8 is involved in transcriptional regulation and may also recruit E3 ubiquitin-protein ligase complexes to proteins targeted for proteasomal degradation. Multiple isoforms of Med8 exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Med8 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-268 representing full length Med8 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-268 representing full length Med8 of human origin |
| Entrez Gene | 112950 |
|---|
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing nuclear localization.
Application
Western blot analysis of Med8 expression in non-transfected: (A) and mouse Med8 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Med8 expression in Hep G2 whole cell lysate.| Application Notes | recommended for detection of Med8 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
