Application
Western blot analysis of NFS1 expression in HeLa (A), Hep G2 (B) and SK-N-MC (C) whole cell lysates.Application
Western blot analysis of NFS1 expression in 293T (A) and HeLa (B) whole cell lysates.| Product Name | NFS1 Antibody (H-170) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | NFS1 (nitrogen fixation 1), also known as NIFS or IscS (cysteine desulfurase), is a member of the class V pyridoxal-phosphate-dependent aminotransferase family. It localizes to the cytoplasm or mitochondrion depending on which form is generated based on cytosolic pH. Highest expression levels of NFS1 are found in heart and skeletal muscle. Lower levels of expression are also found in liver, brain and pancreas. NFS1 is responsible for catalyzing the removal of sulfur from cysteine to form alanine, thereby supplying the inorganic sulfur for iron-sulfur (Fe-S) clusters. Fe-S clusters function as essential cofactors in a wide variety of events, including facilitation of electron transfer processes in oxidative phosphorylation, catalysis of enzymatic reactions in aconitase and dehydratases and maintenance of structural integrity in the DNA repair enzyme endonuclease III. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of NFS1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 288-457 mapping at the C-terminus of NFS1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 288-457 mapping at the C-terminus of NFS1 of human origin |
| Entrez Gene | 9054 |
|---|
Application
Western blot analysis of NFS1 expression in HeLa (A), Hep G2 (B) and SK-N-MC (C) whole cell lysates.
Application
Western blot analysis of NFS1 expression in 293T (A) and HeLa (B) whole cell lysates.| Application Notes | recommended for detection of NFS1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
