Application
Western blot analysis of S-100A3 expression in non-transfected 293T: (A), human S-100A3 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of S-100A3 expression in OVCAR-3 whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skin tissue showing cytoplasmic staining.| Product Name | S-100A3 Antibody (T-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The S-100 protein family consists of a group of calcium-binding proteins that are exclusively expressed in vertebrates and exhibit cell and tissue-specific expression. The expression levels of its members differ in various pathological conditions. The extracellular functions of the S-100 family may include the ability to enhance neurite outgrowth, involvement in inflammation and motility of tumor cells. S-100A3 (S100 calcium binding protein A3), also known as S100E, is a 101 amino acid cysteine-rich protein that belongs to the S-100 family of proteins. Expressed specifically at the inner endocuticle of hair fibers, S-100A3 contains two EF-hand domains and exists as a multimer. S-100A3 binds calcium with low affinity and zinc with high affinity and is believed to play a role in the calcium-dependent differentiation of cuticle cells and in the formation of hair shafts. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of S-100A3 of human origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against S-100A3 of human origin |
|---|---|
| Tissue Specificity | raised against S-100A3 of human origin |
| Entrez Gene | 6274 |
|---|
Application
Western blot analysis of S-100A3 expression in non-transfected 293T: (A), human S-100A3 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of S-100A3 expression in OVCAR-3 whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skin tissue showing cytoplasmic staining.| Application Notes | recommended for detection of S-100A3 of human origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
