Application
Western blot analysis of Unc18-1 expression in non-transfected 293T: (A), human Unc18-1 transfected 293T: (B) and IMR-32 (C) whole cell lysates.Application
Western blot analysis of Unc18-1 expression in non-transfected 293T: (A), mouse Unc18-1 transfected 293T: (B) and IMR-32 (C) whole cell lysates.| Product Name | Unc18-1/2 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Unc18-1, 2 and 3 (also known as Syntaxin binding proteins 1-3, STXBP1-3, UNC18-a–c and Munc18-1–3) are chaperone molecules that block Syntaxin interactions with cognate SNARE (soluble NSF attachment protein (SNAP) receptors) proteins and regulate exocytosis. Unc18-1–3 mRNA is present in RBL-2H3 mast cells, mouse bone marrow derived mast cells (BMMC) and platelets. Unc18-1 Ser 313 is a protein kinase C phosphorylation site. Thr 574 is a cyclin-dependent kinase 5 phosphorylation site that regulates Unc18-1/ Syntaxin 1A interactions. Unc18-1 is phosphorylated on Ser 313 in response to phorbol ester treatment in adrenal chromaffin cells. Unc18-2 co-localizes with Syntaxin 3 at the apical plasma membrane of intestinal, proximal tubule and collecting duct epithelial cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Unc18-1, Unc18-2 and, to a lesser extent, Unc18-3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of Unc18-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of Unc18-1 of human origin |
| Entrez Gene | 6812 |
|---|
Application
Western blot analysis of Unc18-1 expression in non-transfected 293T: (A), human Unc18-1 transfected 293T: (B) and IMR-32 (C) whole cell lysates.
Application
Western blot analysis of Unc18-1 expression in non-transfected 293T: (A), mouse Unc18-1 transfected 293T: (B) and IMR-32 (C) whole cell lysates.| Application Notes | recommended for detection of Unc18-1, Unc18-2 and, to a lesser extent, Unc18-3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
