Application
Western blot analysis of Raver1 expression in MDCK (A) and NIH/3T3 (B) whole cell lysates and mouse liver tissue extract (C).Application
Western blot analysis of Raver1 expression in non-transfected: (A) and human Raver1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Raver1 expression in non-transfected 293T: (A), human Raver1 transfected 293T: (B) and Hep G2 (C) whole cell lysates.Application
Western blot analysis of Raver1 expression in Hep G2 whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining.| Product Name | Raver1 Antibody (I-16) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Raver1, also known as Ribonucleoprotein PTB-binding 1, is a widely expressed 606 amino acid protein that forms complexes with microfilament-associated proteins such as vinculin, metavinculin and å-Actin at microfilament attachment sites. Localized to either the nucleus or cytoplasm, Raver1 regulates alternative splicing events by associating with hnRNP I. With three RNA recognition motifs (RRM) near the N-terminus, Raver1 is thought to function as a potent splicing co-repressor by promotion of exon skipping. In myocytes, Raver1 has been shown to translocate from the nucleus to the cytoplasm, targeting the costamere. Here, it complexes with microfilament-associated proteins during muscle cell differentiation, which suggests that Raver1 may coordinate RNA targeting and processing as required for microfilament anchoring in adhesion sites. There are three isoforms of Raver1 due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Raver1 of human origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against Raver1 of human origin |
|---|---|
| Tissue Specificity | raised against Raver1 of human origin |
| Entrez Gene | 125950 |
|---|
Application
Western blot analysis of Raver1 expression in MDCK (A) and NIH/3T3 (B) whole cell lysates and mouse liver tissue extract (C).
Application
Western blot analysis of Raver1 expression in non-transfected: (A) and human Raver1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Raver1 expression in non-transfected 293T: (A), human Raver1 transfected 293T: (B) and Hep G2 (C) whole cell lysates.
Application
Western blot analysis of Raver1 expression in Hep G2 whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining.| Application Notes | recommended for detection of Raver1 of human origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
