Application
Western blot analysis of OTC expression in Hep G2 whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human intestine tissue showing cytoplasmic staining.| Product Name | OTC Antibody (W-17) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | OTC (ornithine carbamoyltransferase), also known as OTCase, is a 354 amino acid protein that belongs to the ATCase/OTCase family of proteins. Expressed in liver and intestinal mucosa, OTC localizes to the mitochondrial matrix and exists as a homotrimer. Specifically, OTC plays a vital role in the urea cycle, catalyzing the second step in this pathway: the formation of L-citrulline from L-orthinine and carbamoyl phosphate. In humans, the urea cycle is an important pathway to detoxification of ammonia. Mutations in the gene encoding OTC are associated with the X-linked disorder OTCD (ornithine carbamoyltransferase deficiency). OTCD is a disorder of the urea cycle characterized by hyperammonemia. In males, OTCD is fatal, whereas females express variable symptoms. In addition, the OTC gene localizes near a region of the X chromosome that is associated with Duchenne muscular dystrophy, suggesting a possible role in that disease as well. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of OTC of mouse, rat, human and canine origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | raised against OTC of human origin |
|---|---|
| Tissue Specificity | raised against OTC of human origin |
| Entrez Gene | 5009 |
|---|
Application
Western blot analysis of OTC expression in Hep G2 whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human intestine tissue showing cytoplasmic staining.| Application Notes | recommended for detection of OTC of mouse, rat, human and canine origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
