Application
Western blot analysis of human RNF138 transfected 293T whole cell lysate.| Product Name | RNF138 Antibody (W-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation. RNF138 (ring finger protein 138), also known as HSD4 or NARF, is a 245 amino acid protein that contains one RING-type zinc finger and functions as an E3 ubiquitin-protein ligase, playing an important role in protein ubiquitination. Expressed as multiple alternatively spliced isoforms, RNF138 interacts with Nlk and is thought to act as a negative regulator of the Wnt/∫-catenin-mediated signaling pathway. RNF138 may be auto-ubiquitinated and is subject to post-translational phosphorylation, probably by ATM or ATR. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of RNF138 of mouse and human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of RNF138 of mouse and human origin by WB, IP and ELISA |
| Entrez Gene | 51444 |
|---|
Application
Western blot analysis of human RNF138 transfected 293T whole cell lysate.| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
