Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of ALDH1A1 expression in non-transfected: (A) and human ALDH1A1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of ALDH1A2 expression in non-transfected: (A) and mouse ALDH1A2 transfected: (B) 293T whole cell lysates and mouse eye tissue extract (C).Application
Western blot analysis of ALDH1A3 expression in non-transfected 293T: (A), human ALDH1A3 transfected 293T: (B) and Hep G2 (C) whole cell lysates.Application
Western blot analysis of ALDH1A1 expression in non-transfected 293T: (A), human ALDH1A1 transfected 293T: (B) and Hep G2 (C) whole cell lysates.Application
Western blot analysis of ALDH1A1 expression in non-transfected: (A) and mouse ALDH1A1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of ALDH1B1 expression in non-transfected: (A) and human ALDH1B1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of ALDH1/2 expression in Hep G2 whole cell lysate (A) and mouse liver (B) and rat liver (C) tissue extracts.| Product Name | ALDH1/2 Antibody (H-85) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Aldehyde dehydrogenases (ALDHs) mediate NADP+-dependent oxidation of aldehydes into acids during the detoxification of alcohol-derived acetaldehyde; metabolism of corticosteroids, biogenic amines and neurotransmitters; and lipid peroxidation. ALDH1A1, also designated retinal dehydrogenase 1 (RalDH1 or RALDH1); aldehyde dehydrogenase family 1 member A1; aldehyde dehydrogenase cytosolic; ALDHII; ALDH-E1 or ALDH E1, is a retinal dehydrogenase that participates in the biosynthesis of retinoic acid (RA). The major liver isoform ALDH1 localizes to cytosolic space, while ALDH2 localizzes to the mitochondria. The ALDH1A2 (RALDH2, RALDH2-T) gene produces three different transcripts and also catalyzes the synthesis of RA from retinaldehyde. ALDH2 is present in most Caucasians, yet is absent in 50% of Asians. The absence of this enzyme has been linked to alcohol intolerance; and thusly, a reduced risk for alcoholism-related liver disease. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ALDH1A1, ALDH1A2, ALDH1A3 and ALDH2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 186-270 mapping within an internal region of ALDH1A1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 186-270 mapping within an internal region of ALDH1A1 of human origin |
| Entrez Gene | 216 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of ALDH1A1 expression in non-transfected: (A) and human ALDH1A1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of ALDH1A2 expression in non-transfected: (A) and mouse ALDH1A2 transfected: (B) 293T whole cell lysates and mouse eye tissue extract (C).
Application
Western blot analysis of ALDH1A3 expression in non-transfected 293T: (A), human ALDH1A3 transfected 293T: (B) and Hep G2 (C) whole cell lysates.
Application
Western blot analysis of ALDH1A1 expression in non-transfected 293T: (A), human ALDH1A1 transfected 293T: (B) and Hep G2 (C) whole cell lysates.
Application
Western blot analysis of ALDH1A1 expression in non-transfected: (A) and mouse ALDH1A1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of ALDH1B1 expression in non-transfected: (A) and human ALDH1B1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of ALDH1/2 expression in Hep G2 whole cell lysate (A) and mouse liver (B) and rat liver (C) tissue extracts.| Application Notes | recommended for detection of ALDH1A1, ALDH1A2, ALDH1A3 and ALDH2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
