Ribosomes, the organelles that catalyze protein synthesis, are composed of a small subunit (40S) and a large subunit (60S) that consist of over 80 distinct ribosomal proteins. Mammalian ribosomal proteins are encoded by multigene families that contain processed pseudogenes and one functional intron-containing gene within their coding regions. Ribosomal Protein L3, also known as RPL3 or TARBP-B, is a 403 amino acid protein that localizes to the cytoplasm and belongs to the L3P family of ribosomal proteins. Expressed as multiple alternatively spliced isoforms, Ribosomal Protein L3 is able to bind to HIV-1 mRNA, possibly activating HIV-1 protein translation. Like most ribosomal proteins, Ribosomal Protein L3 exists as multiple processed pseudogenes that are scattered throughout the genome.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WBIPIFELISA
recommended for detection of Ribosomal Protein L3 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with other Ribosomal Protein family members also reactive with additional species, including equine, canine, bovine and porcine
Species Reactivity
HumanMouseRat
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Alternative Names
epitope mapping within an internal region of Ribosomal Protein L3 of human origin
Tissue Specificity
epitope mapping within an internal region of Ribosomal Protein L3 of human origin
Western blot analysis of Ribosomal Protein L3 expression in K-562 whole cell lysate.
Application
Western blot analysis of Ribosomal Protein L3 expression in 293T (A) and U-698-M (B) whole cell lysates.
Application Notes
recommended for detection of Ribosomal Protein L3 of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with other Ribosomal Protein family members also reactive with additional species, including equine, canine, bovine and porcine
Additional Information
Form
Liquid
Storage Instructions
For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.