Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Product Name | SHFM3 Antibody (C-17) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | SHFM3 (split-hand/foot malformation type 3), also known as FBXW4 (F-box and WD-40 domain-containing protein 4) or Dactylin, is a 412 amino acid protein that is primarily involved in signaling pathways that are essential for normal limb development. Specifically, SHFM3 promotes ubiquination and degradation of certain phosphorylated proteins. Defects in the gene encoding SHFM3 are the cause of split-hand/foot malformation type 3, an autosomal dominant disorder that is characterized by hypoplasia/aplasia of the central digits, causing variable fusion with the remaining digits. The disease is not a result of a point mutation, but rather a genomic rearrangement resulting in a tandem duplication that contains a disrupted copy of the gene encoding SHFM3, as well as extra copies of two other genes that are linked to limb development. SHFM3 is typically expressed in kidney, brain, lung and liver, with highest expression in fetal brain. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFIHCELISA |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of SHFM3 of human origin by IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of SHFM3 of human origin by IF, IHC(P) and ELISA |
| Entrez Gene | 6468 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
