Application
Western blot analysis of SFRS12 expression in Jurkat (A) and SK-BR-3 (B) whole cell lysates.| Product Name | SFRS12 Antibody (C-16) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Pre-mRNA splicing enhancer elements are short RNA sequences capable of activating weak splice sites in nearby introns that are required for accurate splice site recognition and the control of alternative splicing. Splicing enhancer elements contain specific binding sites for serine/arginine (SR)-rich splicing factors, which include SC35, 9G8, SRp20 and SF2/ASF. The family of SR factors all contain one or more RNA recognition motifs (RRM) and an SR-rich domain. They are not only essential for constitutive splicing, but also regulate splicing in a concentration-dependent manner by influencing the selection of alternative splice sites. Splicing factor arginine/serine-rich 12 (SFRS12), also designated serine-arginine-rich-splicing regulatory protein 86 (SRrp86) or splicing regulatory protein 508 (SRrp508), contains one RRM and two SR-rich domains separated by an unusual glutamic acid-lysine (EK)-rich region. SFRS12 interacts with all core SR proteins as well as other splicing regulatory proteins, such as SAF-B, hnRNP G, YB-1 and p72. SFRS12 both positively and negatively modulates the activity of the SR proteins and its EK domain can inhibit both constitutive and alternative splicing. SFRS12 also interacts with a lysine-rich zinc finger domain-containing protein p18SRP, which is down-regulated in the brain of Alzheimer’s disease (AD) patients. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of SFRS12 of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of SFRS12 of human origin by WB, IP and ELISA |
| Entrez Gene | 140890 |
|---|
Application
Western blot analysis of SFRS12 expression in Jurkat (A) and SK-BR-3 (B) whole cell lysates.| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
