Application
Western blot analysis of HARS2 expression in HeLa whole cell lysate.| Product Name | HARS2 Antibody (C-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The fidelity of protein synthesis requires efficient discrimination of amino acid substrates by aminoacyl-tRNA synthetases. Aminoacyl-tRNA synthetases function to catalyze the aminoacylation of tRNAs by their corresponding amino acids, thus linking amino acids with tRNA-contained nucleotide triplets. HARS2 (histidyl-tRNA synthetase 2), also known as HO3, HARSL or HARSR, is a 506 amino acid protein that localizes to the mitochondrial matrix and belongs to the class II aminoacyl-tRNA synthetase family. Highly expressed in kidney, heart and skeletal muscle with lower levels present in liver and brain, HARS2 functions in the ATP-dependent synthesis of histidyl-transfer RNA, playing an accessory role in the regulation of protein synthesis. The gene encoding HARS2 maps to human chromosome 5, which contains 181 million base pairs and comprises nearly 6% of the human genome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBELISA |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of HARS2 of human origin by WB and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of HARS2 of human origin by WB and ELISA |
| Entrez Gene | 23438 |
|---|
Application
Western blot analysis of HARS2 expression in HeLa whole cell lysate.| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
