Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tissue showing nuclear and cytoplasmic staining of respiratory epithelial cells.Application
Western blot analysis of GR expression in NIH/3T3 nuclear extract.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human skin showing nuclear localization of glucocorticoid receptor.| Product Name | GR Antibody (M-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The glucocorticoid receptor (GR) is a ubiquitously expressed transcription factor that mediates the effects of glucocorticoids. The most abundant isoform is GR a. GR induces or represses the expression of genes in response to glucocorticoids, mediating such processes as apoptosis, cell growth and differentiation. A significant class of genes suppressed by GR is controlled by the transcription factor AP-1. GR has also been shown to be the limiting factor in the induction of gene expression by glucocorticoids. It has been revealed that GR forms a complex with HSP 90, rendering the non-ligand bound receptor transcriptionally inactive. More importantly, mutant GRs lacking the signaling domain remain constitutively active. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of GR &alpha and GR &beta of mouse, rat, and, to a lesser extent, human origin by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of GR &alpha of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of GR α of mouse origin |
| Entrez Gene | 2908 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tissue showing nuclear and cytoplasmic staining of respiratory epithelial cells.
Application
Western blot analysis of GR expression in NIH/3T3 nuclear extract.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human skin showing nuclear localization of glucocorticoid receptor.| Application Notes | recommended for detection of GR &alpha and GR &beta of mouse, rat, and, to a lesser extent, human origin by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM204203